To reconstruct Aβ manufacturing in mouse embryonic fibroblasts, wild-type or mutant Aph1-aL cDNAs ended up inserted into your Eco
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5-fold greater activity than did the WT (Figure 3a). Immunoblotting confirmed the expression amounts of mutant Aph1 and PS1 were just like concentrations within the get more info WT. These effects propose that γ-secretase Using these two Aph1 mutations show Improved protease activity for application.
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